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Our regulatory network analysis identified FOXM1 and KDM4E as signature transcription factor of AF and NP respectively, which might be involved in the regulation of core genes of AF and NP transcriptome. Subsequent interaction network analysis revealed a structured network of extracellular matrix genes in NP compartments. Further, functional annotation clustering analysis revealed the enrichment of receptor signaling pathways genes in AF cells, while NP cells showed high expression of genes related to the protein synthesis machinery. Gene enrichment analysis showed that SFRP1, BIRC5, CYTL1, ESM1 and CCNB2 genes were highly expressed in the AF cells whereas, COL2A1, DSC3, COL9A3, COL11A1, and ANGPTL7 were mostly expressed in the NP cells. Our systematic and comprehensive analyses revealed distinct genetic architecture of human NP and AF compartments and identified 2,196 differentially expressed genes. We defined the transcriptome map of healthy human IVD by performing single-cell RNA-sequencing (scRNA-seq) in primary AF and NP cells isolated from non-degenerated lumbar disc. While various aspects of IDD progression have been reported, the underlying molecular pathways and transcriptional networks that govern the maintenance of healthy nucleus pulposus (NP) and annulus fibrosus (AF) have not been fully elucidated. Intervertebral disc (IVD) disease (IDD) is a complex, multifactorial disease. download software, allows you to listen to music online with no limits, enjoy over 100 million mp3 including recommended hot songs weekly and list of Audio Joiner Join Multiple Audio Files Online Mp3 Cutter click "download" to download the result mp3 file when merge successed. upload your mp3 files, than click "merge" button to merge. concatenating your several music, song, rington, mp3 files to one. 19343 merge mp3 01n meda mp3 joiner 12 audio recorders stepvoice recorder 21 Merge mp3 files, mp3 joiner -online combine several mp3 format files into one mp3 file. no installation, no registration, it's free and easy to use. Free online audio joiner to merge & combine multiple songs into one audio file.
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